BACTERIAL TRANSGLUTAMINASES

摘要

The invention relates to bacterial transglutaminase polypeptide molecules, bacterial transglutaminase nucleic acid molecules, expression vectors and host organisms, in addition to a method for the production of an inactive polypeptide molecule and a method for activating the same. The invention also relates to antibodies against the inventive transglutaminase polypeptide molecules. The aim of the invention is to provide a bacterial transglutaminase enzyme which can be optionally activated in a targeted manner, in addition to providing means and ways of producing and activating said enzyme. The invention further relates to a novel active or activatable bacterial transglutaminase. This is achieved by means of amino and nucleic acid sequences from streptoverticillia that are used for instance in the food, pharmaceutical and cosmetics industry, in order to polymerize substances containing protein and to improve the properties of the final products e.g. with regard to their texture resistance, gel resistance, fracture strength, viscosity, elasticity, taste and odour. The transglutaminases can also be used to immobilize enzymes and antibodies.