发明名称 |
L-lysine producing coryneform bacteria and methods for the production of L-lysine |
摘要 |
L-lysine producing coryneform bacterium (A) with an amplified pyc (pyruvate carboxylase) gene in which at least one of the additional genes dapA (dihydropicolinate synthase), lysC (aspartate kinase), lysE (lysine exporter-carrier) and/or dapB (dihydropicolinate reductase) is amplified, preferably overexpressed. Independent claims are also included for: (a) DNA (I), replicable in coryneform bacteria and preferably recombinant DNA of Corynebacterium origin, which includes a sequence (1; 795 bp), reproduced, that represents the 5'-region of the dapB gene; (b) production of L-lysine by fermenting (A); Escherichia coli K12 strains DH5 alpha /pECTlysE (DSM 12871) or DH5 alpha /pEC7dapBlysE (DSM 12875); C. glutamicum strains DSM5715/pJC23 (DSM 12869), DSM5715aecD::dapA(MA16) (DSM 12867), DSM5715aecD::dapA(MC20) (DSM12868) and DM678 (DSM 12866); and (c) replicable DNA sequences MC20 (79 bp) and MA16 (80 bp), representing mutations of the C. glutamicum dapA promoter. |
申请公布号 |
EP1067193(A1) |
申请公布日期 |
2001.01.10 |
申请号 |
EP20000114502 |
申请日期 |
2000.07.06 |
申请人 |
DEGUSSA AG;FORSCHUNGSZENTRUM JUELICH |
发明人 |
KREUTZER, CAROLINE;MOECKEL, BETTINA, DR.;PFEFFERLE, WALTER, DR.;EGGELING, LOTHAR, DR.;SAHM, HERMANN, PROF.;PATEK, MIROSLAV |
分类号 |
C12N15/09;C07K14/34;C12N1/21;C12N15/53;C12N15/54;C12N15/67;C12N15/77;C12P13/08;C12R1/15 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|