| 摘要 |
<p>The invention concerns a method of identifying transcription factors comprising providing cells with a nucleic acid sequence at least comprising a sequence CACCT as bait for the screening of a library encoding potential transcription factors and performing a specificity test to isolate said factors. Preferably the bait comprises twice the CACCT sequence, more particularly the bait comprises one of the sequences CACCT-N-CACCT, CACCT-N-AGGTG, AGGTG-N-CACCT or AGGTG-N-AGGTG wherein N is a spacer sequence. The identified transcription factor(s) using the method according to the invention comprises separated clusters of zinc fingers such as for example a two-handed zinc finger transcription factor. The present invention further discloses that at least one such zinc finger transcription factor, denominated as SIP1, induces tumor metastasis by downregulation of the expression of E-cadherin. Compounds interfering with SIP1 activity can thus be used to prevent tumor invasion and metastasis.</p> |
