发明名称 |
Cort-pcr assay for the discrimination of endogenous reverse transcriptase activity in eukaryotic cell lines from infectious retrovirus contamination |
摘要 |
The present invention relates to the detection of the presence of reverse transcriptase enzymatic activity in eukaryotic cell lines. Specifically, the present invention comprises a method for discriminating between endogenous reverse transcriptase activity in a substrate cell line (e.g., avian) used as substrates for the manufacture of biological products (including viral vaccines) from exogenous infectious retrovirus by using a combination of transmissibility assay (viral replication) and PBRT assay (RT-PCR). Appropriate levels of viral amplification and number of PCR cycles are determined and employed that permit detection of exogenous infectious RT activity but not endogenous RT activity. |
申请公布号 |
AU5611500(A) |
申请公布日期 |
2001.01.02 |
申请号 |
AU20000056115 |
申请日期 |
2000.06.14 |
申请人 |
AVENTIS PASTEUR |
发明人 |
SCOTT GOEBEL;MAURICE HARMON;JIM TARTAGLIA;WILLIAM LAPPS;FRANCOIS PELLOQUIN;DENNIS W. TRENT |
分类号 |
G01N33/569;C12N15/09;C12Q1/68;C12Q1/70 |
主分类号 |
G01N33/569 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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