Methods and compositions for isolating high molecular weight dna from natural samples

摘要

Methods and compositions are disclosed for isolation and propagation of high molecular weight DNA. Large segments of DNA isolated from samples obtained directly from natural sources such as soil, undersea core samples, fresh and salt water, air, and other sources may be used to examine entire gene clusters and to isolate expression products of previously unknown and uncharacterized or even extinct organisms. Isolation and manipulation of larger DNA segments than has been previously possible is taught herein.