发明名称 BASOPHIL IN BLOOD, AND COMPOSITION AND METHOD FOR DISTINGUISHING BASOPHIL
摘要 PROBLEM TO BE SOLVED: To dissolve erythrocytes and to selectively dissolve a secondary population of leucocytes by using a dissolution reagent composition containing ethoxy long chained amine compound, a 14C quaternary ammonium salt and an acid. SOLUTION: An ethoxy long chained amine compound has a composition represented formula I, in which R is a 12-22C alkyl, an alkenyl or an alkinyl group, (m) and (n) are respectively integers satisfying m+n=20-40, and its concentration is about 0.05-0.8%. The 14C quaternary ammonium salt has a composition represented by formula II, in which R1 is a 14C alkyl, an alkenyl or an alkinyl group, R2 and R3 are each a 1-4C alkyl group, and its concentration is about 0.15-0.4%. As an acid preferably used with these, formic acid, citric acid, oxalic acid and the like, or their mixture can be used, its concentration is about 0.05-1.0%, and pH of the dissolution reagent is about 1.8-2.5. Furthermore, to improve the shelf life, an antiseptic agent having anti-oxidizing characteristic and antibacterial characteristic is added.
申请公布号 JP2000338104(A) 申请公布日期 2000.12.08
申请号 JP20000127402 申请日期 2000.04.27
申请人 COULTER INTERNATL CORP 发明人 YUI RII;JIN LEE;CAROL JO YOUNG
分类号 G01N33/48;C12Q1/06;G01N15/14;G01N33/49;G01N33/50;(IPC1-7):G01N33/48 主分类号 G01N33/48
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