REPORTER MOLECULES AND METHODS FOR ASSAYING THE LIPIDATION STATUS OF A CELL
摘要
The invention relates to reporter molecules comprising at least one detection domain capable of emitting a signal and at least one membrane anchoring domain, which promotes the association of the reporter molecule with a membrane. The degree and rate of membrane association of the reporter molecule is approximated by the signal emitted by the detection domain. This signal provides a marker for the membrane association status of the membrane anchoring portion, or of the reporter molecule as a whole. Treatments or agents may be tested for their ability to alter membrane association or localization by observing or measuring the signal emitted by the detection domain. Thus, the invention provides means and methods for determiming the capability of an agent to interfere at least in part with the distribution of a reporter molecule in a cell. These methods are useful for drug discovery; the phenotypic evaluation of cells, preferably patient cells; and for tailoring a therapeutic program for treating a patient.
申请公布号
WO0073497(A1)
申请公布日期
2000.12.07
申请号
WO2000EP04919
申请日期
2000.05.26
申请人
TIBOTEC NV;VAN ACKER, KOENRAAD, LODEWIJK, AUGUST;DIERINCK, INGE;PAUWELS, RUDI, WILFRIED, JAN
发明人
VAN ACKER, KOENRAAD, LODEWIJK, AUGUST;DIERINCK, INGE;PAUWELS, RUDI, WILFRIED, JAN