| 摘要 |
<p>The present invention relates to a method for screening nucleotide sequences encoding anti microbial peptides comprising the steps of: a) ligating a plasmid with the pool of nucleotide sequences operably linked to an inducible promoter, so as to express a peptide which is an enzyme or a mature peptide of less than 100 amino acid residues, optionally linked to a signal peptide, b) transforming host cells which are sensitive to the peptide with the ligated plasmids, c) screening the transformed host cells so as to select viable cells, d) cultivating the viable cells in the presence of inducer so as to induce expressions of said nucleotide sequence, e) selecting cells according to the effect of the inducer on cell growth, and recovering the nucleotide sequence encoding the peptide from the selected cells. DNA shuffling is emphasized as a method for producing the pool of nucleotides.</p> |
