PREPARATION CONTAINING CELL EXTRACT FOR SYNTHESIZING CELL-FREE PROTEIN AND MEANS FOR SYNTHESIZING CELL-FREE PROTEIN

摘要

A preparation for synthesizing a cell-free protein which contains a cell extract prepared by eliminating from an organism systems relating to the inhibition of self-protein synthesis reactions; an apparatus for synthesizing a cell-free protein provided with a reaction tank for synthesizing the cell-free protein; and a kit to be used therefor. The above preparation is obtained as a product which can be stored at room temperature while sustaining the biological functions of the cell extract. A means for continuously synthesizing a cell-free protein comprising a cell extract from which substances inhibiting self-protein synthesis reactions have been substantially eliminated, involving a procedure selected from among addition, preservation, exchange and discharge of a factor selected from among mRNA serving as a template in the synthesis reactions, an enzyme participating in the energy regeneration system, a substrate and an energy source.