ASSAYS FOR NUCLEOSIDE DIPHOSPHATES AND TRIPHOSPATES
摘要
Nucleoside diphosphates (NDPs) are detected or measured by following the dephosphorylation of the phosphoenzyme form of nucleoside diphosphate kinase (NDPK), and nucleoside triphosphates (NTPs) are detected or measured by following the phosphorylation of NDPK to its phosphoenozyme form. A typical process involves (a) causing NDP in sample to bind to NDPK phosphoenzyme, or causing NTP in sample to phosphorylate NDPK and (b) detecting a change in a charateristic of the enzyme which differs between its phosphorylated and unphosphorylated forms. This may be aided by labelling the enzyme. Quantitative data can be obtained. Both <i>in vivo</i> and <i>in vitro</i> measurements can be made.
申请公布号
WO0068418(A1)
申请公布日期
2000.11.16
申请号
WO2000GB01740
申请日期
2000.05.05
申请人
MEDICAL RESEARCH COUNCIL;BRUNE, MARTIN, HERMANN, KLEMENS;CORRIE, JOHN, EDGAR, THOMAS;WEBB, MARTIN, RONALD
发明人
BRUNE, MARTIN, HERMANN, KLEMENS;CORRIE, JOHN, EDGAR, THOMAS;WEBB, MARTIN, RONALD