发明名称 |
EXPRESSION OF HEPARIN-BINDING PROTEIN IN RECOMBINANT MAMMALIAN CELLS |
摘要 |
The present invention relates to a method for producing a mammalian heparin-binding protein in a mammalian cell that can be cultured under anaerobic conditions after introducing a nucleic acid encoding as heparin-binding protein into said cell with comprising (a) introducing into said mammalian cell a nucleic acid encoding said heparin-binding protein; (b) culturing the cell of step (a) under conditions conducive to expression of said HBP; and (c) recovering said HBP from the culture medium. Furthermore, the invention relates to the recombinant mammalian cell used in the method. |
申请公布号 |
WO0066627(A1) |
申请公布日期 |
2000.11.09 |
申请号 |
WO2000DK00216 |
申请日期 |
2000.04.28 |
申请人 |
NOVO NORDISK A/S |
发明人 |
FLODGAARD, HANS, JAKOB;RASMUSSEN, POUL, BAAD;BJOERN, SOEREN;SVENDSEN, IVAN |
分类号 |
C12N15/09;A61K38/00;C07K14/47;C12N5/10;C12N5/22;C12P21/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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