发明名称 |
Genetic system for controlling background expression of transgene products |
摘要 |
A method and system for controlling the expression of transgene products in specific tissues in a transgenic animal is provided. The method comprises: a) providing a first transgenic parent animal whose genome comprises an F transgene comprising an exogenous gene operatively linked to a promoter that is upregulated by a transactivator protein; b) providing a second transgenic parent animal whose genome comprises i) a second transgene, comprising a second promoter that is downregulated by the transactivator protein and operatively linked to an antisense gene that encodes a sequence which inhibits or reduces processing of the F transgene transcript; and ii) a third transgene comprising a tissue specific promoter operatively linked to a gene encoding the transactivator protein; and c) breeding the first transgenic parent animal with the second transgenic parent animal to provide a transgenic offspring animal whose genome comprises the three transgenes. The present invention also relates to a DNA construct comprising the second transgene and to the second transgenic parent mouse used in the method. |
申请公布号 |
AU4343600(A) |
申请公布日期 |
2000.11.02 |
申请号 |
AU20000043436 |
申请日期 |
2000.04.12 |
申请人 |
THE OHIO STATE UNIVERSITY RESEARCH FOUNDATION, |
发明人 |
CHARLES G. OROSZ;DONGYUAN XIA;GAYLE M. GORDILLO |
分类号 |
A01K67/027;C12N15/85 |
主分类号 |
A01K67/027 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|