SCREENING METHODS BASED ON THE USE OF PROTEIN KINASES

摘要

A method of identifying a compound which inhibits to different extents (a) a host yeast cell protein kinase or kinases and (b) a protein kinase derivable from a source other than the said host yeast cell that is equivalent to the said host yeast cell protein kinase or kinases, wherein a compound is exposed to 1) a first host yeast cell wherein the yeast cell is capable of expressing the said host yeast cell protein kinase or kinases and is not capable of expressing the said equivalent protein kinase and 2) a second host yeast cell wherein the yeast cell is (a) not capable of expressing the said yeast cell protein kinase or kinases and (b) is capable of expressing the said equivalent protein kinase derivable from a source other than the host yeast cell and the effect of the compound on the viability of the said yeast cells is measured, and a compound that affects the viability of the first said yeast cell and the said second yeast cell differently, is identified. The method may be useful in a screen for identifying compounds that inhibit a mammalian or fungal protein kinase. The compounds may be useful in medicine.