发明名称 |
Low temperature expression chitinase cDNAs and method for isolating the same |
摘要 |
A winter wheat derived chitinase cDNA characterised in that the cDNA encodes a protein with chitinase activity at low temperatures. The chitinase polypeptides may have an amino acid sequence corresponding to SEQ ID NOS: 1, 2 or 3. Also claimed is a method cloning such cDNAs using a standard PCR based protocol with specific primers (SEQ ID NOS:4 and 5) to generate fragments for library screening. Transgenic plants transformed with a winter wheat derived chitinase cDNA are claimed. |
申请公布号 |
AU2258500(A) |
申请公布日期 |
2000.10.26 |
申请号 |
AU20000022585 |
申请日期 |
2000.03.24 |
申请人 |
JAPAN AS REPRESENTED BY DIRECTOR GENERAL OF HOKKAIDO NATIONAL A |
发明人 |
AKIRA KAWAKAMI;FUMIHIRO TERAMI |
分类号 |
C12N15/09;C12N9/24;C12N15/29;C12N15/56;C12N15/82 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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