马传染性贫血病毒驴白细胞弱毒疫苗株的全长基因序列

摘要

本发明提供了马传染性贫血病毒(EIAV)驴白细胞弱毒疫苗株含有8258个碱基的前病毒DNA全长基因序列及其结构,其所编码的全部蛋白质的基因序列和氨基酸序列和蛋白质的二级结构,以及该疫苗毒株的调控序列和非必需区序列。这些序列和结构的数据可应用于包括艾滋病毒在内的所有慢病毒属病毒的疫苗研制和使用该属病毒作为载体进行的基因治疗,还可应用于对EIAV的血清学及分子生物学诊断方法及其所需试剂的研制。

主权项

1.马传染性贫血病毒(Equine Infectious Anemia Virus，EIAV)驴白细胞弱毒疫苗株的全长基因序列，其特征在于该基因由8258个核苷酸组成，5’-3’的序列为： 1 TGTGGGGTTT TTATGAGGGG TTTTATAAAT GATTATAAGA GTAAAAAGAA GGGGGCTGAT GCTCTCATAA CCTTGTATAA CCCAAAGGAC TAGCTCATGT 100101 TGCTAGGCAA CTAAACCGCA ATATCCTGTA GTTCCTCTTG CGTTCCGCAT TTGTGACGTT TTAAGTTCCT GTTTTTACAG TATATAAGTG CTTGTATTCT 200201 GACAATTGGG CACTCAGATT CTGCGGTCTG AGTCCCTTCT CTGCTGGGCT AGACTAGCCT TTGTAATAAA TATAATTCTC TGCTAAGTCC CTGTCTCTAG 300301 TTTGTCTTGT TTTCAAGATC TAACAGCTGG CGCCCGAACA GGGACCTGAG GGCGCAGACC CTGCCTGCTG AACCTGGCTG ATCATAGGAT CCCTAGGACA 400401 GCAGAGGAGA ACTTACAGAA GTCTTCTGGA GGTGTTCCTG GCCACAACAC AGGAAGACAG GTAAGATGGG AGACTCTTTG ACATGGAGCA AAGCGCTCAA 500501 GAAGTTAGAG AAGGTGACGG TACAAGGGTC TCAAAAGCTA ACTACTGGTA ACTGTAATTG GGCGCTGAAT TTGGTGGACT TATTCCATGA CACCAATTTT 600601 GGTAAAGAAA AAGACTGGCA ATTAAGGGAC GTCATTCCAT TGTTAGAGGA CGTTTCCCAG ACGTTGTCAG GACAAGAGAG AGAGGCATTT GAAAAAACTT 700701 GGTGGGCAAT AGCTGCCGTT AAGATGGGCT TACAAATTAA TACTGTGAAT GATGCAAAAA CAACATTTTC TATATTAAAA GCCAAGTTTG AAAGAAAGAC 800801 TGCAAATAAT ACCAAAAAGC AGTCTGAGCC CGAGGAAGAA TACCCAATAA TGATTGATGG GGCTGGAAAC AGAAACTTTC GGCCATTAAC ACCCAGAGGA 900901 TATACTACCT GGGTAAATAC TATACAGCAA AACAATCTCT TAAATGAAGC TAGTGTGAAT TTATTTGGTA TTTTATCAGT AGACTGTACT TCTGAGGAAA 10001001 TGAATGCATT TTTGGATGTA GTACCAGGAC AAGCAGGACA AAAACAAGTA CTATTGGATA ATCTTGATAA GATTGCAGAA GAATGGGATC GTAGGCACCC 11001101 GTTGCCAAAT CCTCCATTAG TGGCACCACC ACAAGGGCCT ATTCCCATGA CAGCAAGGTT CATTAGGGGA TTGGGAGTTC CTAGAGAAAG ACAGATGAAA 12001201 CCTGCTTTTG ATCAGTTTAG ACAAACTTAT AGACAATGGA TAATAGAAGC AATGACAGAA GGGATAAAAA TAATGATTGG GAAACCCAAA GCGCAAAATA 13001301 TTAGGCAAGG ACCCAAAGAA CCCTATCCAG AGTTTATAGA CAGATTGCTG TCTCAGATAA AAAGTGAGGG ACATCCGGCT GATATAACTA AATTCCTGAC 14001401 AGACACTTTA ACTATTCAGA ATGCTAATGA TGAATGCAAA AATGCTATGA GACATTTGAG GCCAGAAGAT ACTTTAGAAG AGAAAATGTA TGCATGTAGA 15001501 GATATTGGCA CTATGAGACA AAAAATGGCA TTATTAGCCA AGGCACTTCA AGCAGGATTA GCTGGTCCTA TGAAGGGAGG AATATTTAAA GGGGGACCCT 16001601 TAGGGGCGAA GCAGACATGT TATAATTGTG GAAAACCAGG ACATTTTTCT AGTCAATGTA AAGCACCTAA AATATGTTTT AAGTGCAAAC AGCCAGGACA 17001701 TTTCTCAAAA CAATGTATAA ATGCTCCAAA AAACGGGAAA CAAGGGGCTC AGGGGAGGCC CCAGAAACAA ACTTTCCCTG TGCAGAAGGA GTCAATGAAC 18001801 AAAACACAAA AAGAGGAGAA ACAGCAAGGG ACCTTATATC CAGATTTAAG TCAGATGAAA CAGGAATACA AGATCAAGGA AGAGGAAAAT CAAGAGGATC 19001901 TCAATCTGAA CAGTTTGTGG GAGTAACTTA TAATTTAGAA AAGAGACCAA CTACAATAGT CTTGATTAAT GACACACCCT TAAATGTATT GTTGGACACA 20002001 GGAGCAGACA CATCAGTACT AACTATTGCA CATTGTAATA GGTTAAAGTA TGGAGGAAGA AAATATCAAG GTACAGGTAT TGTTGGGGTT GGAGGTAATG 21002101 TAGAAACATT TTCCACTCCT GTTACAGTGA AAAAGAAAGG AAAACAAATT AAAACTAGAA TGTTAGTAGC AGATATCCCA GTTACTATTT TGGGGCGAGA 22002201 TATTCTTCAA GAATTAGGCG CACAATTACT AATGGCTCAA CTTTCAAAAG AAATAACCCC AAGAGAAATT AAATTAAAAA CAGGCACAGT AGGGCCTAAG 23002301 GTTCCCCAAT GGCCACTTAC TAAAGAGAAG TTGTTAGGTG CTAAAGAAAT AGTCAAAAAA TTGTTGGATG AAGGTAAAAT ATCAGAAGCC AGTGATGATA 24002401 ATCCTTATAA TTCTCCTATA TTTGTAATAA AAAAGAAATC TGGAAAGTGG AGATTATTGC AAGATTTAAG AGAGTTAATT AAGGGTGGTA CAAGTAGAAC 25002501 TGAAATATCC AGAGGATTAC CTCATCCAGG GGGATTAATT AAATGTAATC ATATGACAGT ATTAGATATT GGAGATGCAT ATTTCACTAT ACCATTAGAT 26002601 CCAAAGTTTA GACAATATAC AGCATTTACT GTGCCATCCA TTAATCATCA GGAACCAGAT AAAAGATATG TGTGGAATTG CTTGCCACAA GGTTTTGTGT 27002701 TAAGTCCATA CATATATCAA AAAACATTAC AGGACATATT ACAAGCTTTT AGAGAAAGGC ATCCAGATGT ACAATTATAT CAATATATGG ATGATTTATT 28002801 CATTGGGAGT AATGAATCTA AAAGACAACA TAAGGAACTA GTAGAAGAAT TAAGAGCTAT TCTTTTAGAA AAGGGCTTTG AGACGCCTGG GGATAAATTG 29002901 CAGGAAGAAG CACCCTATAA TTGGCTGGGA TATCAACTTA GTCCAGGCAA TTGGAAAGTA CAAAAGATGC AATTAGAATT GGTAAAAGAG CCAACATTAA 30003001 ATGATGTGCA AAAATCAAAG GGAAATATAA CATGGATGAG CTCAGGGGTT CCTGGATTAA CAGTGAAGCA AATAGCTGCT ACCACTAAAG GTTGCTTAGA 31003101 TTTAAATCAT AAAGGTAGTA GGACCAGAGA AGCCCAAAAA GACTTAGAGG AAATTATTAA AAGTTTCAGA AGCTCAGGAT TCCCATATTA TAACCCAGAA 32003201 GAAGAAGTAA TCTGTGAGAT TGAAATTACT AAAAATTATG AGGCTACTTA TATAATAAAA CAGTCTCAAG GAATAT1GTG GGCAGGAAAG AAAATTATGA 33003301 GGGCTAATAA AGGATGGTCC GCAGCAAAAA ATCTAATGTT ATTGTTACAA CATGTAGCCA CAGAAAGTAT TGTTAGAATT GGAACATGTC CAAAATTTAA 34003401 AGTACCTTTT ACTAAAGAAC AAGTCAAATG GGAAATGGAA AAGGGATGGT ATTATTCATG GCTACCAGAC ATGGTATATT CACATCAAGT TGTTCATGAT 35003501 GATTGGAGAC TGAAATTAGT AGAGCAACCA ACATCTGGTA TAACAATTTA TACTGATGGG GGTAAACAGA ATGAAGAAGG AGTTGCAGCT TATGTGACTA 36003601 GTAATGGGAA AACTAAACAA AAAAGGTTAG GGCCTGTTAC TCATCAAACT GCTGAGAGGA TAGCAATACA AATGGCATTA GAAGATACTG AAGAGACATT 37003701 GGTAAATATA GTAACTGATA GTTACTACTG TTGGAAAAAT ATTACAGAAG GATTAGGGTT AGAAGGACCA GACAGCCCCT GGTGGCCAAT AATTCAAAAT 38003801 ATTAGGGCTA AAGAAATGGT TTATTTTGCT TGGGTACCAG GTCACAAAGG AATATATGGC AATCAATTGG CAGATGAGGC TACTAAAATA ACAGAGGAAA 39003901 TTATGTTAGC ATATCAAGGC ACACAGATTA GGGAAAAAAG AGATGAAGAT GCAGGGTATG ATTTGTGTAT TCCTTATGAC ATAATGATAC CTGTCTCTGA 40004001 GACAAAAGTT ATACCCACAG ATGTAAAAAT ACAGGTACCT CACAAATGTT TTGGATGGGT AACTGGTAAG TCATCAATGG CTAAGCAAGG ATTATTAATC 41004101 AATGGGGGAA TAATTGATGA AGGATACACA GGTGAAATAC AGGTAATTTG TACTAATATT GGAAAGAGTA ACATGAAACT CAGGGAAGGA CAAAAGTTTG 42004201 CACAATTAAT CATATTACAG CATCGATCAA ATGATAAACA AATCTGGGAT GAAAATAAAA CATCTCAAAG GGGAGATAAA GGGTTTGGAA GCACAGGTAT 43004301 ATTTTGGGTA GAGAATATCC AAGAGGCGCA AGATGAACAT GAAAATTGGC ATACATCTCC AAAGATATTG GCAAAAAGAT ATGGGTTACC ATTGACTGTA 44004401 GCTAAACAGA TAACTCAAGA ATGCCCTCAT TGTACTAAAC AAGGATCTGG ACCAGCAGGT TGTGTAATGA GATCTCCTAA TCATTGGCAG GCTGATTGTA 45004501 CACATTTAGA AAACAGGGTA ATAATGACAT TTGTAGAGTC TAATTCAGGA TACATTCATG CTACTCTATT GTCCAAAGAA AATGCCTTGT GTCCTTCATT 46004601 GGCTATTTTG GAATGGGTGA GGTTATTTTC TCCTAAATCT TTACATACAG ACAATGGTAC TAATTTTGTG GCAGAGTCAG TAGCAAATCT GTTGAAATTC 47004701 CTGAAGGTGA CACATACTAC AGGAATACCT TATCACCCAG AGAGCCAAGG CATTGTGGAA AGAGCAAACA GGACATTAAA AGAAAGAATT AAAAGTCATA 48004801 GAGGAAATAC TCAGACACTT GAAGCAGCAT TACAACTTGC TCTCATTACT TGTAACAAAG GGAGGGAAAG TATGGGAGGA CAAACTCCAT GGGAAGTATT 49004901 TATTACTAAT CAGGCTCAAA CAATACATGA AGAACTTTTA TTACAACAAG CACAATCTTC TAAAAAATTT TGTTTTTATA AAATTCCTGG TGAGCATAAT 50005001 TGGAAGGGGC CCACCAGAGT GTTGTGGAAA GGTGATGGAG CAGTAGTGGT CAATGATGAG GAAAAAGGAA TAATTGCTGT GCCTTTAACC AGGACTAAAT 51005101 TATTAATAAG ACCAAATTGA GCATTGTTTC AGGAATCACC ACCAGTCAGC TATCATTGTC AACTGTGTTT CCTGAGATCA TTGGGAATTG ACTACCTTGA 52005201 CAGCTCGCTG AAGAAGAAGA ACAAACAAAG ACAGAAGGCC ATCAGGGAGG AAGACAACCT CAGTATCTTG TTATAAGGTT TGGTGTATGG GATTATTTGG 53005301 TAAAGGGGTA ACATGGTCAG CATTACATTC TATGGGGGTA TCCCAGGGGG AATATCAACC CCTATCACCC AACAAACAGA ATCAACAGAC ACACAGAAAG 54005401 GGGATCATAT GGTATATCAA CCCTATTGTT ATAATGATAG CCATAAAGAA GAAATGGCAG AGACAAGAGA CACAAGATAC CAAGAAGAAA TGAACCGGAA 55005501 AGAAGATAAA GAAGATAAAA GAAAGAATAA CTGGTGGAAG ATAGGTATGT TCTTATTGTG TCTGTTAGAG ATCACTGGAG GATTCCTCTG GTGGTATGAG 56005601 AGGCAACAAC ATTCATATTA TATAAGATTG GTTACAATAG GAGGTAGACT GAATGGTTCA GGAATGACTA GTGCCATAAA ATGTTGGGGT TCATTTCCTG 57005701 GGTGTAGGCC ATTTACTAAC TATTTCAGTT ATGAGACTAA TAGGACTGTT AGTAGAGATA ATAATACTGC TACTCTGTTA GATACTTATC AAAGAGAAAT 58005801 AACAAACATA TACAGGACAT CTTGTGTGGA TAGTGATCAC TGTCAAGAAT ATAAATGTAA GCAAGTACAG TTGAAAAAGA ACAGCAATAA CATTATAATG 59005901 AATAATTGTA GTAACAATAG GTGTGAAGAG TTTTGGGGGT TTAGCTGGTT AGAATGTAAT CAGACAGAAA ATGCAATAAC TATATTGGTC CCAGAAATAG 60006001 AAATACAGCA AAGAAAGAAC ACTTGGATTC CAAAAAGGTG TGAGAAAACT TGGGCTAAGG TAAAACATTG TCCAATGGAT TTATTATATG GTATAAATAA 61006101 AATAAGAATG TGTGTCCAAC CTCCATTCTT TTTGTTTAAA CAGAATGATA CTTCTAATAA TACTAATATT CTCAGTAATT GTGGACCTTT AGTATTTCTT 62006201 GGAATATTTG AGGACAATAA GGCAGCAATC CAGAATGGGA GTTGCACTCT TCACAGGACA AATATTAACA GGCCAGATTA TAGTGGATTT TACCAAGTGC 63006301 CTATATTTTA TATATGCACC TTGACAGGAT TTCAAAGTTG TAATAATGGA TCAATAATTA GTATAATTAT GTATGAGTCT AATAATGTTC AATACTTGTT 64006401 ATGCAATACT AGTAATACTA ATAGTACCAA TAATGCTAAT GTCTCTTGTG TGGTACAAAG TTTTGGAGTG ATAGGACAGG CACATGTGGC ATTGCCCAGA 65006501 AAAAATAAGA GGTTACAATC TCCAAAGTTT GCTCACTATA ATTGCACCAT AAATAATAAA ACAGAGTTAA GGCGATGGCA ATTGGTAAAA ACATCAGGCA 66006601 TCACTCCTTT ACCCATTTCC TCTACAGCTA ATACTGGATT AGTCAGACAC AAGAGAGACT TTGGTATATC TGCTATAATA GCTGCCATTG TAGCTGCTAG 67006701 TGCTATTGCT GCTAGTGCTA CTATGTCTTA TATCGCTTTG ACAGAAGTCA ACAAATTAGA TAGTGTACAA AATCATACTT TTGAAGTAGA GAACAATACT 68006801 ATCAATAACA TAGAGTTAAC AGAAGAGCAA ATTCATATAT TATATGCTAT GGTTCTCCAA ACACATGCAG ATGTTCAATT GTTAAAAGAA CAACAAAAGA 69006901 TTGAGGAAAC ATTTAATTTA ATTGGATGTA TAGAAAGATC ACATACATTT TGTCATACTG GACATCCCTG GAATGAATCA TGGGGTCAGT TAAATGATTC 70007001 TACACAGTGG GATGACTGGG TAGATAAGAT GGAAAATTTA AATCATGATA TATTAACAAC ACTTCATACT GCTAGAAATA ATCTAGAACA ATCTATGATA 71007101 ACTTTCAATA CACCTGACAG TGTAGCACAA TTTGGAAAAA ATATTTGGAG TCATATTGCA AATTGGATTC CTAGATTAGG AGCTTCCATA ATTAAATATA 72007201 TAGTGTTGAT ATTACTTATA TATGTGTTAC TAACCTCTGC ACCTAAGATC CTCAGAGGCC TCTTGACAAC GATGAGTGGT GCAGGATCCT CCGCCAGTCG 73007301 CTACCTGAAG AAAAGATACC ATCACAAACA TGCATCGCGA GGAGACATCT GGGCCCAGGT CCAATATCAT GCGTACCTGG CAGACGAGAC TCATGGCTCA 74007401 GGGGACAAGT CCAACATGCG GAAGCTCTCC AGGAACAACT GGAATGGCGA ATCAGAGGAG TACAACAGAC GACAAAAAAA TTGGAAAAAG TTATTAAAGA 75007501 GATCTGGAGA GAATTACAAT ACACACGAAG ACAACATGGG GACTATGGGT CGTTTGGTGA CTACCGCCGC CGAGAAGAAG AACGTCGGGG TGAATCCTCA 76007601 CCAAGGGTCC TTAACCCTGG AGATTCAAAG CAAAGGAGGA AACATCTATG ACTGTTGCAT TAAGGCTCAA GAAGGAACTC TTGCTATTCC TTGCTGTGGC 77007701 TTCCCACTAT GGCCGTTTTG GGGACTTATA ATCATATTAG AACGCTTGTT GGGATATGGG CTTCGGGAAA TTGCAAAAAT TATAATGATT CTAGGGAAAG 78007801 GACTAAGTAT AATAATTACA GGATTAAGAA AATTATGTGA TTATATTGGG AAAATGCTAA ATCCAGCTAC ATCTCATGTA ACAATGCCTC AATATGATGT 79007901 TTAGAAAAAC AAGGGGGGAA CTGTGGGATT AATATAAGAT TCTTATAAGT GAATATGAAA GTTGCTGATG CTCTCAAGTT GCTGATGCTC TCATAACCTT 80008001 ATGACTAGCT CATGTTGCCA GGCAACTGAA CTGTGATAAC CTTTTGTTCC TCATTATAGT TCCGCTTTTG TATAGTTCCG CTTTTGTGAC GCGTTAAGTT 81008101 CCTGTTTTTA CAGTATATAA GTGCTTATAT TCTGACATTT GGTCACTCAG ATTCTGCGGT CTGAGTCCCT TCTCTGCTGG GCTAGACTAG CCTTTGTAAT 82008201 AAATATAATT CTCTGCTAAG TCCCTGTCTC TAGTTTGTCT TGTTTTCAAG ATCTAACA 8258