HISTOLOGICAL RECONSTRUCTION AND AUTOMATED IMAGE ANALYSIS

摘要

A method for automated image analysis (Fig. 2, 42) of a biological specimen by histological reconstruction, provided is an automated cell image method for analyzing a biological specimen that has consecutively been stained by eithe r an in situ hybridization (ISH) method, or an immunohistochemistry (IHC) meth od or a nucleic acid stain, and counterstained. The method couples composite images in an automated manner for processing and analysis to identify structure in tissue that cannot be captured in a single field of view image (Fig. 2, 44) or a single staining technique, the disclosure provides a metho d for histological reconstruction to analyze many fields of view on potentiall y many slides (Fig. 2, 37) simultaneously.