INDUCTION OF CALLUS OF LIMONIUM SINUATUM, CULTURE AND PROLIFERATION OF CALLUS AND CREATION OF REDIFFERENTIATED PLANT BODY

摘要

PROBLEM TO BE SOLVED: To carry out high frequency mutation breeding, cell fusion breeding, etc., with other related genera by culturing a part of a plant such as Limonium sinuatum in a culture medium containing picloram. SOLUTION: Induction of callus is carried out by culturing a part of leaf cut piece, etc., of a plant body of Limonium sinuatum in a culture medium containing picloram (preferably having 0.1-1.0 mg/L concentration in the culture medium). Furthermore, callus culture and proliferation of Limonium sinuatum is preferably carried out by culturing callus in a culture medium containing picloram and redifferentiated plant body of Limonium sinuatum is preferably created by culturing induced callus or cultured and proliferated callus in a culture medium containing cytokinin (preferably having 0.1-1.0 mg/L concentration in the culture medium) such as benzyladenine or zeatin.