发明名称 |
PRODUCTION OF RECOMBINANT MONELLIN USING METHYLOTROPHIC YEAST EXPRESSION SYSTEM |
摘要 |
The present invention relates to a single-chain monellin-like protein which is stable and which is at least 100-fold sweet as compared to sucrose on the weight basis. The present invention also relates to a nucleic acid encoding said monellin-like protein. Preferably, the nucleic acid further comprises a promoter and a signal sequence for directing expression and secretion of the encoded monellin-like protein in the methylothrophic yeast Pichia pastoris. The present invention further relates to a recombinant Pichia pastoris cell containing the nucleic acid encoding the monellin-like protein, a process for producing the monellin-like protein from the recombinant Pichia pastoris and product of the process.
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申请公布号 |
WO0040603(A3) |
申请公布日期 |
2000.09.21 |
申请号 |
WO1999US29213 |
申请日期 |
1999.12.09 |
申请人 |
GENWAY BIOTECH, INC.;DUAN, LINGXUN |
发明人 |
DUAN, LINGXUN |
分类号 |
C12N15/09;C07K14/415;C07K14/43;C07K19/00;C12N1/19;C12N15/81;C12P21/02;C12R1/84;(IPC1-7):C12N15/29;C12N15/62 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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