METHOD FOR THE SENSITIVE DETECTION OF NUCLEIC ACIDS

摘要

The present invention relates to a method for the specific detection of a nucleic acid A in a sample which comprises the following steps in the given sequence: a) reacting the sample with one or several labelled mononucleoside triphosphates and one or several enzymes which catalyze the production of a labelled nucleic acid B which contains said labelled mononucleoside triphosphates, b) non-thermal denaturation of the reaction mixture subsequent to step a), c) hybridization of the nucleic acid B with a nucleic acid probe C which contains at least one immobilizable group and which is sufficiently complementary to the nucleic acid B, and simultaneous immobilization of the nucleic acid probe C to a solid phase which can bind the immobilizable nucleic acid probe C, d) separating the liquid phase from the solid phase, and e) detecting the nucleic acid hybrid D formed from the labelled nucleic acid B and nucleic acid probe C by detecting the detectable group bound to the solid phase.