| 摘要 |
An electrophoresis separation material, especially for isoelectric focusing, comprising a carrier material to which pH-buffering groups are firmly attached and anode side and cathode sides. At least a first portion of the pH-buffering groups (a) having a pH-dependent charge on a nitrogen atom binding to an sp2-hybridised carbon atom,(b) including groups with pKa>=9.5. One embodiment, the groups in (a) have the structure -(NH)nC(=NH)(NH2)(I), possibly in protonated form, where n is 0 or 1, and the free valence binds to the carrier material via an organic linker. An IEF separation material obtained by polymerisation of a mixture of monomers and containing immobilised buffering groups capable of establishing an immobilised pH-gradient where(1) at least one monomer has the structure CH2=CHR''CONR12R13 (V) where (i) R'' represents hydrogen or methyl; (ii) one or more of R12 and R13 are -((CH2)n'-O)n''-H where n' is 2-3 and n'' is 1-5 while any remaining group R12 and R13, is hydrogen and when n is 3, a hydrogen on the middle carbon in (CH2)n may be replaced with hydroxy; and (2) the immobilised pH gradient extends to pH>10. |
