发明名称 |
Assay using marked microbial host cell strains |
摘要 |
An assay is provided for determining the presence of contaminating microbial host cells in a culturing vessel containing a microbial host cell strain that utilizes more than one carbohydrate source as a substrate comprising: (a) culturing the microbial host cell strain in the vessel, wherein the strain comprises nucleic acid encoding a polypeptide of interest and is genetically marked so as not to utilize a carbohydrate source as a substrate; (b) plating an isolated sample of culture from step (a) onto culture media supplemented with a carbohydrate source not utilized by the host cell strain as a substrate; (c) incubating the plated cells at a temperature and for a time sufficient for any positive colony to grow to a detectable level; and (d) detecting whether any colonies are growing on the supplemented culture media.
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申请公布号 |
US6114137(A) |
申请公布日期 |
2000.09.05 |
申请号 |
US19970949443 |
申请日期 |
1997.10.14 |
申请人 |
MCFARLAND, NANCY C.;SWARTZ, JAMES R. |
发明人 |
MCFARLAND, NANCY C.;SWARTZ, JAMES R. |
分类号 |
C07K14/65;C12N1/20;C12Q1/22;(IPC1-7):C12Q1/06;C12Q1/04;C12N15/00 |
主分类号 |
C07K14/65 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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