发明名称 |
Nucleotide sequence encoding a 52 kDa Ro/SSA autoantigen |
摘要 |
Complementary DNA encoding a 52 kDa form of a protein present in the human Ro/SSA ribonucleoprotein complex has been cloned. A lambda gt11 cDNA library made from human thymocyte mRNA was screened with serum from a SLE patient and two immunoreactive clones were isolated. These clones reacted with other patient sera which had anti-52 kDa Ro/SSA antibodies and with affinity purified anti-52 kDa Ro/SSA antibodies. Moreover, affinity purified antibodies eluted from fusion proteins of the isolated clones reacted only with the 52 kDa protein of lymphocytes in the Western blot. Ro/SSA RNAs were also precipitated with these affinity purified antibodies, further confirming that the clones encode a 52 kDa Ro/SSA antigen. The sequence differs from the previously reported 60 kDa Ro/SSA gene.
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申请公布号 |
US6111088(A) |
申请公布日期 |
2000.08.29 |
申请号 |
US19920945830 |
申请日期 |
1992.09.16 |
申请人 |
OKLAHOMA MEDICAL RESEARCH FOUNDATION |
发明人 |
FRANK, MARK BARTON;ITOH, KAZUKO |
分类号 |
A61K38/00;C07K14/47;C07K16/18;(IPC1-7):C07H21/04;C12Q1/68;C12N1/08;C08G63/82 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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