| 摘要 |
The present invention provides proteins of a general formula: (Xaa)nTpp(Xaa)m, wherein Tpp is selected from the outer membrane proteins of Treponema pallidum Tpp15, Tpp17 and Tpp47, and any immunoactive fragments and derivatives thereof that can bind to human immunoglobulins; Xaa represents a natural amino acid residue, wherein at least one of Xaas which are nearest to the two ends of Tpp and at least one terminal Xaa of the formula are selected from the group consisting of Lys, Arg and Trp; n and m represent independently an integer from 1 to 10, providing n+m>/=2. A novel method that assays anti-Treponema pallidum antibodies using the proteins, particularly ba the double-antigen-sandwich immunoassay is provided. An immunoassay kit for detecting human anti-Treponema pallidum antibodies by the method of present is also provided. |
