| 摘要 |
PROBLEM TO BE SOLVED: To inexpensively transform a bud yeast or the like by utilizing a segment consisting of transcriptional factor gene YAP1 and adventitious PGK promoter and using an enhanced resistance ability to medicines due to the enhancement in the expression of a transcriptional factor as an indicator. SOLUTION: By utilizing a segment consisting of transcriptional gene YAPI and adventitious PGK promoter and using a vector capable of confirming the introduction of a gene to a bud yeast by using an enhanced resistance ability to medicines due to the enhancement in the expression of a transcriptional factor as an indicator, the bud yeast which can use an inexpensive medicine as a selective marker and selects the objective gene efficiently to use in the various investigations and the production of liquors or breads, particularly the bud yeast which is used in the production of useful proteins due to a recombinant DNA using Saccharomyces cerevisiae as a host, is transformed.
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