A METHOD FOR SELECTING AND PRODUCING VACCINE AND DIAGNOSTIC PREPARATIONS

摘要

There is disclosed a method for selecting and producing vaccine and diagnostic preparations against a specific pathogenic organism, wherein a non-human vertebrate is immunized with a preparation of the pathogenic organism, samples containing the antibodies to the pathogen-preparation are taken from immunized vertebrates, a gene library of the genome of the pathogenic organism is produced, which gene library is capable of being expressed in host cells, the pathogen polypeptide sequences-expessing gene library-host cells being selectable as regards their binding to an antibody, antigenic polypeptides are identified by contacting the gene library with the antibody-containing sample taken, and those clones of the gene library are selected whose expressed polypeptides bind to an antibody, and the identified antigenic polypeptides are finished to a vaccine or diagnostic preparation.