摘要 |
PCT No. PCT/EP95/02703 Sec. 371 Date Sep. 29, 1997 Sec. 102(e) Date Sep. 29, 1997 PCT Filed Jul. 7, 1995 PCT Pub. No. WO96/02633 PCT Pub. Date Feb. 1, 1996Novel acid-stable and thermo-stable enzymes having alpha -1,4 hydrolytic activity and a alpha -1,6 hydrolytic activity which are derived from strains of the genus Sulfolobus. These enzymes are capable of expressing high levels of alpha -1,4 hydrolytic activity, including the maximum alpha -1,4 hydrolytic activity thereof, at highly acidic pHs of between about 2.5 and about 4.5. These alpha -amylases are further capable of expressing high levels of alpha -1,4 hydrolytic activity, including the maximum alpha -1,4 hydrolytic activity thereof, at high temperatures of between about 90 DEG C. and about 120 DEG C. Particularly disclosed herein are such enzymes which are derived from strains of the species S. acidocaldarius and, in particular, Sulfolobus acidocaldarius DSM 639. Modified starch degradation (liquefaction and saccharification) processes using these novel enzymes are also disclosed herein.
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