发明名称 DEVELOPMENT OF RHAMNOSE MACCONKEY(RMAC) CULTURE MEDIUM WHICH IS SELECTIVE SEPARATION CULTURE MEDIUM FOR ENTEROHEMORRHAGIC ESCHERICHIA COLI O26 AND RHAMNOSE MACCONKEY CULTURE MEDIUM CONTAINING CEFIXIME AND POTASSIUM TELLURITE ADDED THERETO (CT-RMAC)
摘要 PROBLEM TO BE SOLVED: To obtain the subject culture medium capable of selectively separating enterohemorrhagic Escherichia coli O26 and useful for a rapid testing method, etc., with a high precision for infectious diarrhea by adding rhamnose as a saccharide component to a basal culture medium for detecting the Escharichia coli. SOLUTION: This selective separation culture medium is obtained by adding rhamnose as a saccharide component in an amount of 0.1-50 g based on 1,000 ml of a culture medium, further, as necessary, adding a tellurite such as potassium tellurite in an amount of 0.1-25 mg based on 1,000ml of the culture medium and further adding an antibiotic substance such as cefixime capable of inhibiting the growth of intestinal floras in an amount of 0.01-1.0 mg based on 1,000 ml of the culture medium to a basal culture medium for detecting the Escherichia coli. The resultant culture medium is capable of culturing a sample and selectively separating enterohemorrhagic Escherichia coli O26 to be a causative bacterium of infectious diarrhea at a frequency next to enterohemorrhagic Esherichia coil O157 and is useful for a rapid testing method, etc., with a high precision for infectious diarrhea.
申请公布号 JP2000210076(A) 申请公布日期 2000.08.02
申请号 JP19990016814 申请日期 1999.01.26
申请人 AICHI PREFECTURE 发明人 SAITO MAKOTO;HIRAMATSU REIJI;MIWA YOSHIO;MATSUMOTO MASAKADO
分类号 C12Q1/04;C12N1/20;C12R1/19;(IPC1-7):C12N1/20 主分类号 C12Q1/04
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