Method for the determination of glycosylated haemoglobin

摘要

The determination of glycosylated and non glycosylated hemoglobin in blood samples is carried out by using boron-containing dyestuff conjugates and measuring levels of glycosylated hemoglobin and total hemoglobin at different wavelengths. The determination of glycosylated and non glycosylated hemoglobin in a probe comprises: (1) optional hemolyzation of the probe in order to release cell-bound hemoglobin; (2) contacting the probe with an excess of signal-forming molecules, so that some of the molecules bind to glycosylated hemoglobin; (3) removing any unbound signal-forming molecules; and (4) measuring the amount of bound signal-forming molecules to establish the amount of glycosylated hemoglobin, then measuring the total amount of hemoglobin and subtracting the first value from the second to find out the level of non-glycosylated hemoglobin. An Independent claim is included for an analytical test element that utilizes this technique.