| 摘要 |
PURPOSE:To obtain the subject phospholipid-analog compound capable of quickly lowering the high blood pressure by injection as well as oral administration, keeping the activity over a long period and free from side action by extracting fruit body and mycelia of Grifola frondosa with an organic solvent. CONSTITUTION:The objective phospholipid-analog compound expressed by formu la I (R1 and R2 are 16-20C fatty acid residue having one or two double bonds; R3 is group of formula II) and free from nitrogen can be produced by mixing Grifola frondosa with ether, heat-treating at 50 deg.C for about 3 hrs, concentrating the extract, treating with acetone to collect an acetone-insoluble component, adsorbing the component to a column filled with silicic acid, eluting with a mixture of chloroform-methanol-water(103:50:8) to collect 5 fractions, selecting a fraction having strong hypotensive action from the above fractions and purify ing the fraction by liquid chromatography using Cosmoseal 5C18 column as a carrier. |
