IMMORTALIZED CELL LINES AND METHODS OF MAKING THE SAME

摘要

The present invention relates to methods of immortalizing various primary ce ll cultures, including pituitary cells, neurons, beta islet cells, glial cells, corneal epithelial cells and follicular stellate cells. The primary cells ar e transfected with a vector containing an establishment oncogene, resulting in non-transformed immortalized cells. The primary cells and/or the subsequent immortalized cells are cultured in a defined media containing one or more environmental factor(s) that control the proliferation and/or differentiatio n of the cells.