摘要 |
The culture and preservation of Lactobacillus clearans require special considerations because the titer readily decreases. There is thus an urgent need for culture media and preservatives to prevent such a decrease in bacterial titer during both subculture and storage. The present invention relates to a culture medium in which at least one or more of sodium sulfide and ammonia is decreased by Lactobacillus clearans during culture with the addition of at least one or more of sodium sulfide and aqueous ammonia, as well as to a method for preserving Lactobacillus clearans in which at least one or more of a sulfur-containing amino acid, ovalbumin, bile powder, trehalose, raffinose, dead yeast cells, chlorella, rice bran, bran, soybean milk, and carrot juice is or are present as a preservative around the bacteria.
|