| 摘要 |
<p>PURPOSE: Provided are methods for purifying carbohydrates, including oligosaccharides, nucleotide sugars, and related compounds, by use of ultrafiltration, nanofiltration and/or reverse osmosis CONSTITUTION: A carbohydrates are purified away from undesired contaminants such as compounds present in reaction mixtures following enzymatic synthesis or degradation of oligosaccharides. A method of purifying a carbohydrate compound from a feed solution containing a contaminant, the method comprising contacting the feed solution with a nanofiltration or reverse osmosis membrane under conditions such that the membrane retains the carbohydrate compound while a majority of the contaminant passes through the membrane, wherein the carbohydrate compound is selected from the group consisting of: sialyl lactosides; sialic acid; LnNT; LNT-2; NeuAcα(2#3)Galss(1#4)(Fucα1#3)Glc(R1)ss1-OR2, wherein R' is OH or NAc; R2 is a hydrogen, an alkoxy, a saccharide, an oligosaccharide or an aglycon group having at least one carbon atom; and Galα(1#3)Galss(1#4)Glc(R1)ss-O-R3, wherein wherein R' is OH or NAc; R3 is -(CH2)n-COX, with X=OH, OR4, -NHNH2, R4 being a hydrogen, a saccharide, an oligosaccharide or an aglycon group having at least one carbon atom, and n=an integer from 2 to 18.</p> |
