发明名称 |
Producing low binding hydrophobic surfaces by treating with a low HLB number non-ionic surfactant |
摘要 |
Hydrophobic polymer surfaces whose level of protein binding is less than about 50-80 ng/cm2 are achieved by: (1) applying to a hydrophobic polymer surface a coating solution composed of a solvent and a non-ionic surfactant having a HLB number of less than 5 and at least one hydrophilic element which can extend into an aqueous solution; and (2) drying the surface to remove the solvent and thereby bring the surfactant into direct contact with the hydrophobic polymer. The combination of a low HLB number and the drying step have been found to produce low binding surfaces which can withstand multiple washes with water and/or protein-containing solutions. Alternatively, the low binding surfaces can be produced by applying the non-ionic surfactant to mold surfaces which contact molten polymer and form the polymer into a desired shape, e.g., into a multi-well plate, a pipette tip, or the like. Further, the low binding surfaces may be produced by incorporating non-soluble, non-ionic surfactants having an HLB number of less than or equal to 10 into a polymer blend prior to molding the article.
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申请公布号 |
US6093559(A) |
申请公布日期 |
2000.07.25 |
申请号 |
US19970918354 |
申请日期 |
1997.08.26 |
申请人 |
CORNING INCORPORATED |
发明人 |
BOOKBINDER, DANA CRAIG;FEWKES, JR., EDWARD JOHN;GRIFFIN, JAMES ARTHUR;SMITH, FRANCES M.;TENNENT, DAVID L. |
分类号 |
B05D5/08;B01L99/00;B05D3/02;C07H21/00;C08J7/04;C08J7/06;G01N33/543;(IPC1-7):C12N9/00;G01N33/53;C07K17/00 |
主分类号 |
B05D5/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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