| 摘要 |
<p>The invention provides modified reverse transcriptase polypeptides (Types I, II, and III), along with polynucleotides encoding such polypeptides, vectors containing such polynucleotides and host cells transformed with those polynucleotides. The modified RTs typically exhibit improved stability and/or improved solubility, relative to naturally occurring reverse transcriptases. The modified RTs are also found in a variety of forms, such as monomers as well as both homo- and hetero-multimers. The modified RTs may be used in any one or more of the methods known to benefit from reverse transcriptase activity, such as cDNA synthesis, and amplification techniques such as PCR and RAMP.</p> |
