HEPHATIC DISEASE DIAGNOSIS METHOD USING MULTIPLE DETECTION STRIP FOR HEPHATIC DISEASE RELATED MARKER

摘要

PURPOSE: A multiple detection strip simultaneously detects hephatic 1disease related markers in the blood or urine sample of patient. Color change is easily detected by bare eyes or automated analyzer can be used to quantify the concentration of markers to diagnose disease. CONSTITUTION: GPT detection strip is prepared from drying of membrane treated with Tris bufferd solution containing pyruvate oxidase, peroxidase, magnesium chloride, thiamine, pyrophosphate chloride, phosphate salt, alanine, alpha-keto-glutaric acid and 3,3',5,5'-tetramethy benzydine. GOT detection stripe is prepared from drying of membrane treated with Tris buffered solution containing oxalacetic acid carboxylase, pyruvate oxidase, peroxidase, magnesium chloride, thiamine, pyrophosphate chloride, phosphate salt, L-aspartate, alpha-keto-glutaric acid and tetramethy benzydine. Albumin detection strip is prepared from drying of membrane treated with solution containing citric acid, bromocresol green, ethyl orange, Brij 35 and Tween 20. Total protein detection strip is prepared from drying of membrane treated firstly with distilled water containing copper sulfate, treated secondly with NaOH then, thirdly treated with tartrate, KI and polyethylene glytol. Bilirubin detection strip is prepared from drying of membrane treated with ethanol containing phenylsulfoxide and dichlorobenzene diazonium naphthalein sulfonate and then treated with distilled water containing naphthalein sulfonate sodium salt, oxalic acid and lauryl sulfate salt. Urobilinogen detection strip is prepared from drying of membrane treated with methanol containing phosphoric acid, citric acid, 4-methoxy benzene diazonium tetrafluroborate.