METHOD FOR THE CELLULAR HIGH-THROUGHPUT-DETECTION OF NUCLEAR RECEPTOR LIGAND INTERACTIONS

摘要

The invention relates to fusion proteins comprising at least three domains, whereby the first domain mediates a membrane localisation of the fusion protein in a cellular context and the second domain is or might be provided with a ligand binding function of a nuclear receptor and a third domain can activate a signal path in a cell, whereby the signal path connects to a RAS protein. The invention is characterised in that a third domain cannot activate a RAS protein-connected signal path of a cell in spite of membrane localisation when the binding is missing or when the ligand is bonded to the second domain. The invention also relates to cells which comprise fusion proteins of that kind, to assay tests using said cells which can detect specific interactions between ligand binding sections of nuclear receptors and ligands. The invention further relates to kits for using said assays.