| 摘要 |
The invention relates to a method and device for diagnosing allergy symptoms by detecting in vitro immunoreactions. According to the inventive method, a binding protein is applied to a substrate on which antibodies of a patient that is to be examined are immobilized by coupling to the binding protein, and are subsequently exposed to an antigen solution containing the antigens which trigger the allergy for the patient. The substrate is configured as a reflective solid substrate on which the binding protein is applied in a monomolecular, surface-active layer. After an incubation time, a growth in layer thickness of the protein antibody layer is ellipsometrically determined, said growth being caused by an immunoreaction between the antibody and the antigen.
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