CONTROL OF GENE EXPRESSION IN EUKARYOTES

摘要

A chemically inducible gene expression system is described. A chimaeric gene having a first sequence comprising a promoter and a regulator polypeptide is linked with a second sequence comprising a promoter and a coding or non-codi ng sequence. Expression of the target gene of the second sequence is controlled by the regulator polypeptide which is acted upon an inducer. The inducer is a chemical compound, such as OHP, which acts upon the OHP responsive regulator polypeptide, which can be obtained from Rhodococcus sp. V49. Various domain regions and complementary response elements are also described.