| 摘要 |
PROBLEM TO BE SOLVED: To obtain a pair of new nucleic acid probes capable of determining the bacterial strains of a composite microorganism system by labeling plural nucleic acids capable of reacting with target base sequences, respectively, with a pair of fluorescent pigment molecules capable of causing a fluorescence resonance energy transfer phenomenon. SOLUTION: A pair of nucleic acid probes are obtained by labeling two oligonucleotides with a pair of fluorescent pigment molecules capable of causing a fluorescence resonance energy transfer(FRET) phenomenon. The two oligonucleotides can be hybridized with target base sequences, respectively. Therein, when the two nucleic acid probes are hybridized with the target base sequences, respectively, one of a pair of the fluorescent pigment molecules labels one of the mutually faced molecular ends of the two oligonucleotides, and the other labels the chain of the other oligonucleotide, in order that the fluorescent pigment molecules labeling the nucleic acid probes most effectively cause the fluorescence resonance energy transfer(FRET) phenomenon. A pair of the new nucleic acid probes thereby enable to simply and rapidly measure the amounts of specific bacterial stains in the composite microorganism system. |
