| 摘要 |
Amplification and cycle sequencing primer sets have been developed for the detection and analysis of antibiotic resistance-associated mutations in defined regions of the rpoB (rifampin), katG (isoniazid), oxyR-ahpC PR (isoniazid), mabA (isoniazid), rpsL/s12 (streptomycin), 16S/rrs (streptomycin), embB (ethambutol), pncA (pyrazinamide), gyrA (ciprofloxacin) and 23S (azithromycin) genes of <i>Mycobacterium tuberulosis</i>. These primers can be used in a method for detection and characterization of <i>Mycobacterium tuberculosis</i> present in a sample. The method includes the steps of obtaining a sputum sample suspected of containing M. tuberculosis, performing a first sequencing procedure, with or without prior amplification, on the sample to detect the presence of <i>M. tuberculosis</i>, and if present to evaluate the rpoB, katG, rpsL/s12 and 23S genes for the presence of antibiotic-resistance inducing mutations; and (c) if <i>M. tuberculosis</i> is detected in step (b), performing a second sequencing procedure, with or without prior amplification, on the sample to evaluate the additional genes for the presence of antibiotic-resistance inducing mutations. |
