| 摘要 |
PURPOSE: A general method for achieving selective photo-activation of one or more molecular agents with a high degree of spatial control, is provided, that is capable of meeting the diverse needs of the medical diagnostic industry. CONSTITUTION: A method for the imaging of a particular volume of plant or animal tissue,is provided, wherein the plant or animal tissue contains at least one photo-active molecular agent. The method includes the steps of treating the particular volume of the plant or animal tissue with light sufficient to promote a simultaneous two-photon excitation of the photo-active molecular agent contained in the particular volume of the plant or animal tissue, photo-activating at least one of the at least one photo-active molecular agent in the particular volume of the plant or animal tissue, thereby producing at least one photo-activated molecular agent, wherein the at least one photo-activated molecular agent emits energy, detecting the energy emitted by the at least one photo-activated molecular agent, and producing a detected energy signal which is characteristic of the particular volume of plant or animal tissue.
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