PURIFICATION OF ECHINOCANDIN CYCLOPEPTIDE COMPOUNDS
摘要
A method is described for separating and purifying a wide variety of fermentation cyclopeptide products containing at least one protonatable amino group (including the deacylated Echinocandin-type compounds) from their fermentation or mixed broths and partially purified process streams by adsorbing the mixture onto a hydrophobic, reversed phase chromatographic media and eluting with a continuous linear acetic acid gradient ranging from 0.1 % acetic acid to 10.0 % acetic acid by volume in water. A process for removing tripeptide-aldehyde by-products from the fermentation products by means of a derivatizing agent is also described.
申请公布号
WO0034315(A2)
申请公布日期
2000.06.15
申请号
WO1999US29008
申请日期
1999.12.08
申请人
ELI LILLY AND COMPANY;DOBBINS, JOHN, ROBERT;KROEFF, EUGENE, PAUL;VICENZI, JEFFREY, THOMAS
发明人
DOBBINS, JOHN, ROBERT;KROEFF, EUGENE, PAUL;VICENZI, JEFFREY, THOMAS