| 摘要 |
Heterocyclylalkene-substituted quinolinium and pyridinium derivatives are new. The substituted quinolinium derivatives are compounds (Ia) and (Ib) and the substituted pyridinium derivatives are compounds (IIa) and (IIb). Z = group of formula (i)-(iii): X = O; S; Se; NR; C(CH3)2; or CH=CH; R1-R15: at least one is an isothiocyanate, isocyanate, mono- or dichlorotriazine, aziridine, sulfonyl halide, N-hydroxysuccinimide ester, imido ester, glyoxal, maleimide, iodoacetamide or phosphoramidite group capable of covalent bonding to a chromophore and optionally bonded via a spacer -(CH2)m- and at least one can be an ionizable or ionized group, e.g. SO3<->, PO3<->, COO<-> or NR<3+> ; m = 1-18; n (not shown in formulae, but assumed to define the unit in square brackets) = 1-3; or R11 and R12 are bridged to form formula (iv) or (v) when n = 2 and formula (vi) or (vii) when n = 3: A-G = R1-R15 or A, B and C = O; S; C(CN)2; or N(R); R = aliphatic or aromatic group (optionally reactive), e.g. (CH2)nCOOH or (CH2)NH 2; or D = Cl; or an aromatic or aliphatic ring optionally substituted with R<1>-R15; or R1-R10 = higher condensed aromatic or heterocyclic rings. Independent claims are also included for: (A) the preparation of compounds (Ia)-(IIb); and (B) a system for the qualitative or quantitative determination of proteins, nucleic acids, oligomers, DNA, RNA, biological cells, lipids, polymers, pharmaceuticals and polymer particles by the covalent coupling of compounds (Ia)-(IIb) to these substances via OH, NH2 or SH groups carried by the latter.
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