a nucleic acid sequence encoding a lactoferrin variant or portion thereof which has modified iron binding capacity

摘要

The nucleic acid sequence encoding a lactoferrin variant or a portion thereof which has a modified iron binding capacity. The portion is further defined to comprise a sequence corresponding to at least one iron-binding site of lactoferrin. The sequence corresponding to the iron-binding site of the lactoferrin comprises a mutation or deletion of one or more amino acids selected from Asp 396, Tyr 93, Tyr 193 and His 254 in the amino terminal lobe and Asp 396, Tyr 436, Tyr 529 and His 598 in the carboxy terminal lobe. A vector and the transformed eukaryotic cell thereof comprising the above nucleic acid sequence as defined above, a promoter and transcription and translation initiation and termination sequences is used for the expression of human lactoferrin.