METHOD AND TEST KIT FOR EVALUATING THE OXIDATIVE MODIFICATION OF SUBSTANCES CONTAINING PROTEIN BY MEASURING THEIR ANTIOXIDATIVE ACTIVITY

摘要

The invention relates to a method and test kit for the quantitative assessment of the oxidative modification of substances containing protein and the oxidative stress in living organisms, by measuring the antioxidative properties of their protein-containing components, such as body fluids or tissues. According to the invention, the samples are examined in a free-radical generating system after separation of low-molecular antioxidants, for example by gel chromatography, and the measurement results are compared with those of a sample of identical amino acid composition but which has not been subjected to oxidative stress (standard value). The method for measuring the antioxidative activity of proteins is based on the fact that some amino acids which have not been modified by oxidation do not have said property as components of proteins but acquire it during oxidative stress.