METHODS FOR THE PRODUCTION OF TCR GAMMA DELTA T CELLS
摘要
The method for obtaining and expanding TcR gamma delta <+> T cells in culture is described. The method involves: 1) culturing cells from a sample containing TcR gamma delta <+> T cells or precursors thereof in a first culture medium comprising a T cell mitogen and at least two cytokines and 2) culturing the cells obtained in step 1) in a second culture medium comprising at least two cytokines. Preferably, the method comprises 1) culturing the cells in a first culture medium comprising (a) a T cell mitogen, (b) interleukin-2 and (c) interleukin-4; and 2) culturing the cells obtained in step 1) in a second culture medium comprising (i) interleukin-2 and (ii) interleukin-4 to obtain TcR gamma delta <+> T cells. The TcR gamma delta <+> T cells obtained by the method can be used in a variety of experimental, therapeutic and commercial applications.
申请公布号
WO0026347(A1)
申请公布日期
2000.05.11
申请号
WO1999CA01024
申请日期
1999.11.04
申请人
HEMOSOL INC.;BELL, DAVID, NICHOLSON;SKEA, DANNA, LYNN;HEDGE, PHYLLIS, ROBIN
发明人
BELL, DAVID, NICHOLSON;SKEA, DANNA, LYNN;HEDGE, PHYLLIS, ROBIN