发明名称 |
PROCESS FOR REPRODUCING HUMAN GRANULE-COLONY STIMULATING FACTOR(HG-CSF) IN TYPE OF INCLUSION BODY BY GEL FILTRATION COLUMN CHROMATOGRAPHY |
摘要 |
PURPOSE: A process for reproducing a human granule-colony stimulating factor(hG-CSF) is provided which can reduce a reaction volume and obtain a high yield of the hG-CSF by using an inclusion body of the hG-CSF, so it is easy to produce the hG-CSF in a great quantity CONSTITUTION: The process comprises the steps of: suspending the inclusion body of the hG-CSF expressed from a recombinant strain which is cultivated in a solution containing DL-Dithiotheritol(DTT) and sodium dodecyl sulfate(SDS); adjusting pH of the culture solution to an alkali condition; passing the culture solution through a gel filtration column chromatography equilibrated with a buffer solution containing SDS and Cu¬2+; and recovering a fraction of the reproducing hG-CSF.
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申请公布号 |
KR20000021608(A) |
申请公布日期 |
2000.04.25 |
申请号 |
KR19980040784 |
申请日期 |
1998.09.30 |
申请人 |
YUHAN CO., LTD. |
发明人 |
LEE, JONG WUK;YU, GWANG HYEON;JUN, JAE HYEON;PARK, SE CHEOL;NAM, JEONG HYEON;LEE, HO JIN |
分类号 |
C12P21/00;(IPC1-7):C12P21/00 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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