The invention provides diacylglycerol acyl transferase (DAGAT) proteins, wherein said proteins are active in the formation of triacylglycerol from fatty acyl and diacylglycerol substrates. In one aspect, Mortierella ramanniana DAGAT proteins have been isolated and have molecular weights of between approximately 36 and 37kDa as measured by SDS-PAGE. The invention also provides novel DAGAT polynucleotide and polypeptide sequences and to methods of producing such polypeptides using recombinant techniques. In addition, methods are provided for using such sequences to alter triacylglycerol levels in plants and to treat diseases associated with altered DAGAT activity or expression.
申请公布号
WO0001713(A9)
申请公布日期
2000.04.20
申请号
WO1999US15243
申请日期
1999.06.30
申请人
CALGENE LLC;LARDIZABAL, KATHRYN, DENNIS;HAWKINS, DEBORAH;THOMPSON, GREGORY, A.
发明人
LARDIZABAL, KATHRYN, DENNIS;HAWKINS, DEBORAH;THOMPSON, GREGORY, A.