| 摘要 |
<p>A live, attenuated measles virus suitable for incorporation in a measles vaccine is prepared by the serial culture at 35 DEG - 38 DEG C. on chorioallntoic membrane of avian eggs in ovo until established, of an already attenuated measles virus adapted to grow on avian embryo tissue culture, harvesting the established virus an subjecting it to serial culture of the same type at 28 DEG - 32 DEG C. until the required degree of attenuation has been achieved. The virus obtained may be incorporated into conventional parenteral vaccines which may contain a stabilizer e.g. lactose or lactose glutamate.ALSO:A live, attenuated measles virus suitable for incorporation in a measles vaccine is prepared by the serial culture at 35 DEG -38 DEG C. on chorioallantric membrane of avian eggs in ovo until established, if an already attenuated measles virus adapted to grow on avian embryo tissue culture, harvesting the established virus and subjecting it to serial culture of the same type at 28 DEG -32 DEG C until the required degree of attenuation has been achieved. The virus obtained may be incorporated into conventional parenteral vaccines which may contain a stabilizer e.g. lactose or lactose glutamate.</p> |
