发明名称 |
FUSIONS OF SCAFFOLD PROTEINS WITH RANDOM PEPTIDE LIBRARIES |
摘要 |
The invention relates to the use of scaffold proteins, particularly green fluorescent protein (GFP), in fusion constructs with random and defined peptides and peptide libraries, to increase the cellular expression levels, decrease the cellular catabolism, increase the conformational stability relative to linear peptides, and to increase the steady state concentrations of the random peptides and random peptide library members expressed in cells for the purpose of detecting the presence of the peptides and screening rand om peptide libraries. N-terminal, C-terminal, dual N- and C- terminal and one o r more internal fusions are all contemplated. Novel fusions utilizing self- binding peptides to create a conformationally stabilized fusion domain are also contemplated.
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申请公布号 |
CA2345215(A1) |
申请公布日期 |
2000.04.13 |
申请号 |
CA19992345215 |
申请日期 |
1999.10.08 |
申请人 |
RIGEL PHARMACEUTICALS, INC. |
发明人 |
BOGENBERGER, JAKOB MARIA;PEELLE, BEAU ROBERT;ANDERSON, DAVID |
分类号 |
C12N15/00;C07K14/435;C07K19/00;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N7/00;C12N7/01;C12N9/02;C12N9/86;C12N15/10;C12N15/12;C12N15/62;C12Q1/68;(IPC1-7):C12N15/10 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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